Quick-DNA

Quick-DNA kits are commercial nucleic acid extraction systems from Zymo Research. They isolate high-quality DNA from biological samples rapidly. Furthermore, they deliver purified, PCR-ready genomic and pathogen DNA in under 10 minutes from blood, tissue, cells, swabs, saliva, and environmental samples.

The extraction workflow uses a silica-based spin column format. Samples lyse in a chaotropic buffer that denatures proteins and disrupts cell membranes. DNA then binds selectively to the silica membrane under high-salt conditions. Wash steps remove proteins, salts, lipids, and enzymatic inhibitors. These inhibitors would otherwise interfere with downstream PCR, sequencing, and hybridization applications. Furthermore, elution in low-salt buffer releases purified DNA for immediate use or storage. The entire process completes in 4 to 10 minutes. This is significantly faster than phenol-chloroform extraction, which requires 1 to 2 hours and hazardous solvent handling.

Quick-DNA Miniprep kits handle 10 to 100 µL blood samples and tissue pieces up to 25mg. They yield 1 to 20 µg purified genomic DNA per preparation. Quick-DNA Microprep kits extend sensitivity to low-input samples below 50,000 cells. These suit forensic touch DNA samples and environmental swabs with minimal biological material. Additionally, Quick-DNA FFPE kits address formalin-fixed paraffin-embedded tissue sections. A specialized lysis step reverses crosslinking and recovers amplifiable DNA from archived pathology samples.

DNA purity on Quick-DNA eluates reaches A260/A280 ratios of 1.8 to 2.0. This indicates low protein contamination. Moreover, A260/A230 ratios above 2.0 confirm low chaotropic salt carryover — predicting successful performance in qPCR, NGS library preparation, and STR profiling.

These kits serve Pakistan’s forensic DNA laboratories at PFSA and FIA, Shaukat Khanum molecular pathology programs, hospital diagnostic units, university molecular biology departments in Lahore and Karachi, and pharmaceutical QC laboratories.

Tactical Supply Pakistan supplies Quick-DNA extraction kits for forensic profiling, clinical diagnostics, NGS library preparation, and research DNA isolation procurement across Pakistan.